Plant-based meat analogues aggravated lipid accumulation by regulating lipid metabolism homeostasis in mice

To determine the effects of plant-based meat analogues on metabolic health and possible mechanisms, mice were fed with a real pork diet (AP), beef (AB), analogue (PP) (PB) for 68 days. Compared meat, increased food energy intake, body weight, white fat liver weight caused adipocyte hypertrophy, hepatic lipid droplet accumulation, more severe inflammatory responses in mice. Metabolomics revealed that altered composition serum metabolites, which regulated metabolism homeostasis. The PB upregulated gene expression related to synthesis, lipolysis differentiation while PP lipolysis-related genes but downregulated differentiation-related adipose tissue. Meanwhile, both diets influx- synthesis-related oxidation-related liver. specific metabolite biomarkers may affect accumulation mainly by direct pathways or indirect amino acid metabolism, protein digestion absorption, bile secretion, aminoacyl-tRNA biosynthesis, neuroactive ligand-receptor interaction ABC transporters pathways. These findings provide new insight into understanding differences nutritional functions analogues.